fa اثر اینتر فرون بتا در بروزHLA-Gدر سطح سلولهای منوسیت در دیابت تیپ یک Abstract Background and objectives: Dendritic Cells are the most important of antigen presenting cells with an effective role in immune tolerance. This study, aims to clarify the role of IFN- &beta; in induction on dendritic cells derived by monocyte in diabetes type1 to evaluate the T cells response to beta cell specific antigenic molecule. Material and Methods: In this research, peripheral blood mononuclear cells were isolated by phiCole and then dendritic cells generated from blood monocytes ,in Seven days, by adding granulocyte-monocyte colony stimulating factor and interleukin-4 with or without IFN-beta. MRNA was extracted by dendritic cells and cDNA was produced by reverse transcriptase enzyme. Then, Specific polymerase chain reaction for HLA-G was performed. In addition, Tcell proliferation with a mixed Leukocytic reaction evaluated between dendritic cell and T by means of MTT. Results: based on the results, IFN-&beta; induces HLA-G molecule on dendritic cells. In addition, T cell proliferation responses in mixed leukocyte culture show significance difference between Case and control p<0.05. T cell proliferation was inhibited in their co-culture system affected by IFN-&beta; Conclusion: In this study, we show that dendritic cells-treated IFN-&beta; with expression of HLA-G molecule inhibited T cell proliferation and so, our results suggest that some of the IFN- &beta; regulatory effects with expression of HLA-G can probably prevent from beta cell destruction. Key words: dendritic cells, Interferon Beta, Human Leukocytic Antigen-G. Key words: dendritic cells, Interferon Beta, Human Leukocytic Antigen-G 0 0 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-44&slc_lang=fa&sid=1 S Abedian سعید عابدیان abedianlab@yahoo.co.uk 100319475328460013118 100319475328460013118 Yes Immunologist, Assistant Professor ،دانشگاه علوم پزشکی مازندران A.M Mirabi عراز محمد میرابی 100319475328460013119 100319475328460013119 No دانشگاه پزشکی مازندران MR Parsaee محمد رضا پارسایی 100319475328460013120 100319475328460013120 No مرکز بهداشت دانشگاه علوم پزشکی مازندران