fa اثر ضد باکتریایی عصاره متانولی گیاه انجبار( Polygonum bistorta) بر رشد برخی از باکتری ها تحقيقي Original Paper Abstract Background and Objective: Because of increased resistance to antibiotics, side effects of chemical drugs and importance of medicinal plants, we aimed to assess the antibacterial effects of methanolic extract of the Polygonumbistorta plant on the E. coli (ATCC 15224), Ps. aeruginosa (ATCC 25619), B. subtilis (ATCC 6633) and Stap. Aureus (ATCC 25923). Material and Methods: After preparing the extract, its antibacterial effect was assessed via gel diffusion method, using disk / well diffusion methods to determine MIC and MBC Results: MIC of methanolic extract was 78 µg/ml for E. coli, 63×103 µg/ml for Pseudomonas aeruginosa, 39 µg/ml for Bacillus subtilis and 31×102 µg/ml for Staphylococcus aureus Conclusion: In spite of resistance of gram-negative bacteria to chemical agents, polygonum bistorta methanolic extract could inhibit the growth of E.coli and P. aeruginosa. Key words: Antibacterial, Bistorta, Escherichia Coli, Pseudomonas Aeruginosa Antibacterial, Bistorta, Escherichia Coli, Pseudomonas Aeruginosa 41 47 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-223&slc_lang=fa&sid=1 T Ghelich طاهره قلیچ 100319475328460012771 100319475328460012771 No MSc of Microbiology, Islamic Azad University, Tonekabon, Iran M Hashemi Karouei سیدمسعود هاشمی کروئی mssepid@gmail.com 100319475328460012772 100319475328460012772 Yes Assistant Professor of Mycology, Department of Microbiology, Islamic Azad University, Tonekabon, Iran I Gholampor Azizi عیسی غلامپور عزیزی 100319475328460012773 100319475328460012773 No Assistant Professor of Mycology, Department of Biology, Islamic Azad University, Tonekabon, Iran