en انگل شناسی Parasitology Research Article Research Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> <i>Trichomonas vaginalis</i> (<i>T. vaginalis</i>) causes human trichomoniasis, a common type of protozoan vaginitis. Due to the increasing incidence of drug-resistant trichomoniasis, new pharmacological research is needed. The aim was to investigate the activity of <i>Ferula pseudalliacea</i> (<i>F. pseudalliacea</i>) against <i>T. vaginalis</i> and to perform a preliminary phytochemical analysis of its extracts.<br> <b>Methods:</b> Essential oil and various extracts of <i>F. pseudalliacea</i> roots, including n-hexane, ethyl acetate, and methanol, were obtained. Susceptibility testing of the plant products was performed on five <i>T. vaginalis</i> isolates using the microtiter plate method. Minimum lethal concentration (MLC) and growth inhibitory percent (GI%) of sub-MLC concentrations were reported after 24- and 48-hour exposures. Phytochemical screening of the extracts was carried out using a standard procedure.<br> <b>Results:</b> The antitrichomonal effect of the plant products depended on time and concentration, with the greatest effect observed after 48 hours of exposure. The essential oil and n-hexane extract of <i>F. pseudalliacea</i> demonstrated remarkable activity with MLC of 250 &micro;g/ml, followed by the ethyl acetate (MLC=500 &micro;g/ml) and methanol extract (MLC=1000 &micro;g/ml), with GI% 92.8, 50.6, 85.2, and 42.8, respectively. The bioactive constituents of the extracts were coumarins, terpenoids, steroids, phenols, tannins, and glycosides.<br> <b>Conclusion</b>: The results of this study demonstrated in vitro antitrichomonal properties of <i>F. pseudalliacea</i>. Therefore, further studies are needed to investigate the potential antitrichomonal activity of its bioactive constituents.</span></span></div> Antitrichomonal agents, Essential oil, Phytochemicals, Trichomonas vaginalis, Ferula pseudalliacea 25 28 http://mlj.goums.ac.ir/browse.php?a_code=A-10-252-2&slc_lang=en&sid=1 Zarifeh Akbari zarifehakbari94@gmail.com 100319475328460031627 100319475328460031627 No Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran Dara Dastan d.dastan@umsha.ac.ir 100319475328460031628 100319475328460031628 No Department of Pharmacognosy, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran; Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran Mohammad Fallah fallah@umsha.ac.ir 100319475328460031629 100319475328460031629 No Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran Amir Hossein Maghsood a.h.maghsood@umsha.ac.ir 100319475328460031630 100319475328460031630 No Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran Mohammad Matini matini@umsha.ac.ir 100319475328460031631 100319475328460031631 Yes Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran