en ميکروب شناسي Microbiology تحقيقي Original Paper <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><span new="" roman="" times=""><b>Background:</b> Herpes simplex viruses (HSV-1 and HSV-2) are a common cause of dermal, oral, and genital infections worldwide. Rapid diagnosis of infected patients will prevent transmission to uninfected individuals. Therefore, this study aimed to detect HSV-1 and HSV-2 by real-time polymerase chain reaction (RT-PCR) in patients from Golestan, Iran.</span><br> <span new="" roman="" times=""><b>Methods:</b> In this cross-sectional study, 645 suspected HSV patients hospitalized in the health centers of Golestan Province were selected between 2015 and 2020. Demographic data were collected, and DNA was extracted from the samples. Detection of HSV-1 and HSV-2 was performed by RT-PCR.</span><br> <span new="" roman="" times=""><b>Results:</b> Of the 645 samples tested by RT-PCR, 9 samples (1.39 %) were found to be positive for HSV-1/HSV-2, and the rest of the samples (98.6%) were negative. Of the 9 positive samples, 8 cases (88.8%) were HSV-1, of which 7 cases (77.7%) were from the cerebrospinal fluid (CSF) and 1 case (12.5%) from the vagina. HSV-2 was seen only in 1 vaginal specimen (11.1%).</span><br> <b><span new="" roman="" times="">Conclusion</span></b><span new="" roman="" times="">: Early diagnosis of these viral infections is very important as it can lead to early treatment and avoid further symptoms and even death. Thus, molecular approaches can provide an option for quicker diagnosis.</span></span></span></div> Molecular diagnostic techniques,Simplexvirus,Real-time PCR 8 9 http://mlj.goums.ac.ir/browse.php?a_code=A-10-856-4&slc_lang=en&sid=1 Masoomeh Rezanezhadi m.rezanezhadi@gmail.com 100319475328460032021 100319475328460032021 No Kavosh Medical Laboratory, Research and Development Unit, Gorgan, Iran Fahimeh Azadi fahimeh.azadi@ymail.com 100319475328460032022 100319475328460032022 No . Department of Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran. Laboratory Research Center, Golestan University of Medical Sciences, Gorgan, Iran Shayan Marhamati marhamatysh@gmail.com 100319475328460032023 0009-0008-6889-8765 No Department of Biochemistry, Golestan University of Medical Sciences, Gorgan, Iran Hamid Reza Joshaghani hr_joshaghani@yahoo.com 100319475328460032024 100319475328460032024 Yes Laboratory Research Center, Golestan University of Medical Sciences, Gorgan, Iran